Application

Anti-Nitric Oxide Synthase, Inducible antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Inducible nitric oxide synthase (NOS2) produces nitric oxide (NO), which is expected to be lethal to malaria parasites in vitro.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-Nitric Oxide Synthase, inducible is specific for nitric oxide synthase (NOS), derived from activated mouse macrophage RAW 264.7 cells (iNOS, 130 kDa), but does not recognize NOS derived from brain tissue (bNOS) and endothelial cells (eNOS). The NOS isoform characterized in macrophages is a 130 kDa protein, also known as macrophage NOS (macNOS; mNOS), NOS2, or inducible NOS (iNOS). iNOS is an interferon (IFN)-γ-inducible protein. This gene is located on human chromosome 17q11.

Immunogen

synthetic peptide corresponding to amino acids 1126-1144 of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS) conjugated to KLH. The immunogen sequence is highly conserved in rat iNOS and diverges from the human iNOS.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-Nitric Oxide Synthase, Inducible is specific for nitric oxide synthase (NOS), derived from activated mouse macrophage RAW 264.7 cells (iNOS, 130 kDa), but does not recognize NOS derived from brain tissue (bNOS) and endothelial cells (eNOS). By immunoblotting of an activated mouse macrophage RAW 264.7 cells extract, the staining of the iNOS band is specifically inhibited by the immunizing peptide.